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Fig. 2. Regulation of spatiotemporal patterning by protein synthesis in artificial cells. (A) Representative illustration of the experimental system. Symbols indicate the same elements shown in Fig. 1C. (B) Patterns of MinC localization and its relationship with the MinDE concentration. Scale bars indicate 10 μm. (C) Time-development of frequencies of each pattern induced by MinD or MinE (or the N-terminal mutant of MinE) synthesized in artificial cells. Concentrations of DNA and proteins used are described in the figure.