Fig. 4. Direct observation of changes in spatiotemporal patterning in artificial cells by protein expression. (A) MinD was synthesized from 1 nM DNAminD in artificial cells, 16 min intervals. (B) MinD was synthesized from 5 nM DNAminD in artificial cells containing 1 μM purified MinE, 20 s intervals. (C) MinE was synthesized from 1 nM DNAminE in artificial cells containing 1 μM purified MinD, 20 s intervals. (A–C) Scale bars indicate 20 μm.
