Figure 6.

Platelets are increased in the lungs of mice with bleomycin‐induced PH. (a–d) Representative CD41 staining in 3‐week‐old mice treated with IP (a) PBS‐20 × magnification, (b) PBS‐40× magnification, (c) bleomycin‐20× magnification, or (d) bleomycin‐40× magnification, black filled scale bars = 100μm, grey filled scale bars = 50μm. (e) Interstitial platelets in neonatal mice following IP PBS or bleomycin treatment, **p < .005 by unpaired t test, n = 5 PBS (4M,1F), n = 7 Bleo (5M, 2F). (f) PF4 levels in whole lung homogenates from neonatal mice following IP PBS or bleomycin treatment, *p < .05 by unpaired t test, n = 8 PBS (6M, 2F), n = 8 Bleo (3M, 5F). PBS, phosphate‐buffered saline; PF4, platelet factor 4; PH, pulmonary hypertension