Figure 4.

Ectopic overexpression of circNT5E promotes NSCLC cell growth, proliferation and migration. Stable A549 cells (A–E) or primary human NSCLC cells (Pri-Ca-1/-2/-3, F–I) with the lentiviral circNT5E expression construct (LV-circNT5E-Line-1/2, two lines) or the empty vector (LV-C) were cultured for applied time periods, circNT5E expression was tested by qPCR assays (A and F), cell growth (B), proliferation (C, D, G and H) and migration (E and I) were tested by the assays mentioned in the text. Error bars stand for mean ± standard deviation (SD, n=5). * P <0.05 vs. “Pare”/“LV-C” cells. Experiments in this figure were repeated five times, and similar results were obtained.