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Macrophages increase during DO. (A, B) RT-qPCR for CD68 mRNA (A) and ELISA for CD68 protein (B) in regenerating bone tissue. (C, D) FACS for CD68+ cells in regenerating bone tissue by representative flow charts (C), and by quantification (D). DP-day 0: day 0 of distraction phase, CP-day 0: day 0 of consolidation phase, CP-day 28: day 28 or consolidation phase. *p<0.05. NS: non-significant. N=8.
