Table 1.

hiHh isolates used in this study

Isolate	Anatomical site	Country of origin	Year of isolation	Haemin (X-factor) dependence*	NAD (V-factor) dependence*	Haemin biosynthesis pathway†	Genome reference
60819_B_Hi1	BAL	Australia	2010	−	+	C5, PP-dependent, O2-independent	[18]
60824_B_Hi4	BAL	Australia	2010	−	+	C5, PP-dependent, O2-independent	[18]
60971_B_Hi3	BAL	Australia	2012	−	+	C5, PP-dependent, O2-independent	[18]
60982_B_Hi1	BAL	Australia	2012	−	+	C5, PP-dependent, O2-independent	[18]
65117_B_Hi3	BAL	Australia	2011	−	+	C5, PP-dependent, O2-independent	[18]
65151_B_Hi4	BAL	Australia	2011	−	+	C5, PP-dependent, O2-independent	[18]
839_HINF	BAL	USA	2013	Unknown	Unknown	C5, PP-dependent, O2-independent	[24]
CCUG 11096	Pleural fluid	Sweden	1981	−	+	C5, PP-dependent, O2-independent	[3]
CCUG 15949	Eye	Sweden	1984	−	+	C5, PP-dependent, O2-independent	[3]
CCUG 30218	Cerebrospinal fluid	Sweden	1992	−	+	C5, PP-dependent, O2-independent	[3]
CCUG 31732	Ascitic fluid	Sweden	1993	−	+	C5, PP-dependent, O2-independent	[3]
CCUG 66565	Sputum	Sweden	2014	Unknown	Unknown	C5, PP-dependent, O2-independent	This study
F0629	Oral cavity	USA	2015	Unknown	Unknown	C5, PP-dependent, O2-independent	This study
PN24	Urine	Denmark	2004	−	+	C5, PP-dependent, O2-independent	[3]

BAL, Bronchoalveolar lavage.

*, Recorded phenotype.

†, Aminolevulinic acid biosynthesis occurs using the C₅ pathway [63]; coproporphyrinogen III conversion to protohaem is protoporphyrin-dependent, and occurs in an oxygen-independent manner [63].