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. 2020 Mar 2;26:e920981-1–e920981-12. doi: 10.12659/MSM.920981

Figure 2.

Figure 2

miR-195-5p is lowly-expressed in GC cells and downregulation of miR-195-5p reverses the effects of propofol treatment. (A) Differentially expressed miRNAs in SGC-7901 cells with or without propofol treatment evaluated using microarray analysis; (B) 5 miRNAs with most significant differential expression in SGC-7901 cells with or without propofol treatment measured using RT-qPCR. (C) Differential expression of miR-195-5p between GC tissues and normal tissues predicted via the online software (http://starbase.sysu.edu.cn/panMirDiffExp.php). (D) After miR-195-5p inhibitor transfection, miR-195-5p expression in SGC-7901 cells measured using RT-qPCR. (E, F) Migration (E) and invasion (F) of SGC-7901 cells detected using Transwell assays and observed under a microscope (200×). (G) Protein levels of Snail, vimentin and E-cadherin in SGC-7901 cells detected using western blot analysis. In panels D, E, and F, data were analyzed using one-way ANOVA, while in panels B and G, data were analyzed using 2-way ANOVA, and Tukey’s multiple comparisons test was applied for post hoc test; compared to the NC group, * P<0.05, ** P<0.01; repetition=3. GC – gastric cancer; RT-qPCR – reverse transcription quantitative polymerase chain reaction; miR – microRNA; GAPDH – glyceraldehyde-3-phosphate dehydrogenase.