Upregulation of Snail promotes SGC-7901 cell invasion and migration. (A, B) mRNA (A) and protein (B) expression of Snail in SGC-7901 cells after pcDNA3.1-Snail transfection measured using RT-qPCR and western blot analysis, respectively. (C, D) Migration (C) and invasion (D) of SGC-7901 cells following Snail upregulation detected using Transwell assays and observed under a microscope (200×). (E) Protein levels of vimentin and E-cadherin in SGC-7901 cells following Snail upregulation determined using western blot analysis. In panel E, data were analyzed using 2-way ANOVA, while in the rest panels, data were analyzed using one-way ANOVA, and Tukey’s multiple comparisons test was applied for post hoc test; compared to the pc group, * P<0.05, ** P<0.01; repetition=3. RT-qPCR – reverse transcription quantitative polymerase chain reaction.