Table 1.
Gene | Sequence |
---|---|
miR-1827 | F: 5′-ACTAGTTATAACCCTAGGAATTTAGACAACC-3′ |
R: 5′-AAGCTTACATCATTACTCCCATCCCTTAC-3′ | |
miR-195-5p | F: 5′-CTGGAGCAGCACAGCCAATA-3′ |
R: 5′-AGCTTCCCTGGCTCTAGCA-3′ | |
miR-96-5p | F: 5′-AAGCTTACATCATTACTCCCATCCCTTAC-3′ |
R: 5′-CCGGAATTCATCTGCTGGCCCAGCTCAGATTG-3′ | |
miR-93-5p | F: 5′-ACACTCCAGCTGGGTCCTGTACTGAGCTGCCC-3′ |
R: 5′-CTCAACTGGTGTCGTGGA-3′ | |
miR-106b-5p | F: 5′-TGCGGCAACACCAGTCGATGG-3′ |
R: 5′-CCAGTGCAGGGTCCGAGGT-3′ | |
U6 | F: 5′-CTCGCTTCGGCAGCACA-3′ |
R: 5′-AACGCTTCACGAATTTGCGT-3′ | |
Snail1 | F: 5′-TATGCTGCCTTCCCAGGCTTG-3′ |
R: 5′-ATGTGCATCTTGAGGGCACCC-3′ | |
GAPDH | F: 5′-GGTGGTCTCCTCTGACTTCAACA-3′ |
R: 5′-GTTGCTGTAGCCAAATTCGTTGT-3′ |
RT-qPCR – reverse transcription quantitative polymerase chain reaction; miR – microRNA; GAPDH – glyceraldehyde-3-phosphate dehydrogenase.