Figure.5.

Tumor-on-a-chip filters and analyzers. A. A circulating tumor cell (CTC) sorter to capture single cells from flows. The channel curvature generated centrifugal force to push cells into side chambers. (reproduced with permission from ref. 120) B. A filter to capture CTC clusters. Single cells pass through and only cell clusters are captured with the balance of force based on triangular blocker tips. (reproduced with permission from ref. 121) C. The strategy of CTCs isolation on photocleavable ssDNA-encoded antibody conjugates attached to a channel substrate. CTCs could then be released by photoirradiation for single-cell secretomic profiling. (reproduced with permission from ref. 122) D. Tumor cell metabolism inspector. This system had a microfluidic network for media and drug injection, a cell culture chamber, a micro-solid-phase extraction column for sample pretreatment, and an electrospray ionization source for mass spectrometry detection. (reproduced with permission from ref. 123)