Table 1.
Fluorescence intensity data from plate reader analysis of alamarBlue-treated HEK-293, HaCaT, and A431 cells after treatment with dL5**-cetuximab +MG-2I and photoirradiation. Numbers shown are averages and standard deviations, in arbitrary units, from 5 replicates for each condition. Background fluorescence (media + alamarBlue only) has been subtracted out. Irr. = cells irradiated at 640nm. Non-irr. = cells not irradiated. Ester/2I only = only fluorogen, no FAP/antibody. Cetux+Ester/2I = antibody and indicated fluorogen.
| HEK-293 cells | ||||
|---|---|---|---|---|
| Irr. | Non-irr. | |||
| Avg. | StdDev. | Avg. | StdDev. | |
| Neg. ctrl. | 20420 | 3273 | 20997 | 8501 |
| Ester only | 15206 | 4080 | 16790 | 3229 |
| 2I only | 18512 | 2912 | 20633 | 9015 |
| Cetux+Ester | 16882 | 3158 | 20088 | 3802 |
| Cetux+2I | 16946 | 2957 | 19855 | 4996 |
| HaCaT cells | ||||
| Irr. | Non-irr. | |||
| Avg. | StdDev. | Avg. | StdDev. | |
| Neg. ctrl. | 24870 | 1813 | 30172 | 5507 |
| Ester only | 27844 | 1673 | 37122 | 12212 |
| 2I only | 24552 | 2331 | 30150 | 7381 |
| Cetux+Ester | 25932 | 959 | 30473 | 2935 |
| Cetux+2I | 17724 | 1174 | 25108 | 6241 |
| A431 cells | ||||
| Irr. | Non-irr. | |||
| Avg. | StdDev. | Avg. | StdDev. | |
| Neg. ctrl. | 20365 | 1771 | 34047 | 16013 |
| Ester only | 20910 | 1557 | 21852 | 4806 |
| 2I only | 18931 | 1371 | 25351 | 5603 |
| Cetux+Ester | 18380 | 1574 | 23188 | 2619 |
| Cetux+2I | 10427 | 1096 | 32145 | 8698 |