Table 1.

Inter-laboratory study sample characteristics

Study ID	Isolate species	Sequencing method	Carbapenemase gene	Median depth of coverage	Comment
A-1	Klebsiella pneumoniae	NEBNext Ultra II+NextSeq 150 bp PE	OXA-48-like	190.2×	Exact duplicate of A-2
A-2	Klebsiella pneumoniae	NEBNext Ultra II+NextSeq 150 bp PE	OXA-48-like	190.2×	Exact duplicate of A-1
B-1	Enterobacter cloacae complex	NEBNext Ultra II+NextSeq 150 bp PE	OXA-48-like	1.4×	Very low coverage duplicate of B-2
B-2	Enterobacter cloacae complex	NEBNext Ultra II+NextSeq 150 bp PE	OXA-48-like	142.9×	High coverage duplicate of B-1
C-1	Klebsiella oxytoca	Nextera DNA +HiSeq 100 bp PE	OXA-48-like	37.4×	Same original isolate as C-2
C-2	Klebsiella oxytoca	NEBNext Ultra II+NextSeq 150 bp PE	OXA-48-like	156.4×	Same original isolate as C-1
D	Klebsiella pneumoniae	NEBNext Ultra II+NextSeq 150 bp PE	NDM	83.5×
E	Escherichia coli	Nextera DNA +HiSeq 100 bp PE	IMP	20.6×
F	Citrobacter freundii	NEBNext Ultra II+NextSeq 150 bp PE	VIM	32.5×
G	Acinetobacter baumannii	NEBNext Ultra II+NextSeq 150 bp PE	OXA-23-like and OXA-51-like	22.2×

PE, Paired end.