FIGURE 1.

Single cell suspensions from matched periodontitis-affected and healthy gingival tissues were prepared and stained for UV Zombie, CD45, Lin (B220, CD19, Gr1, and Ter119), HLA-DR, CD16, CD14. (A,B) Representative counter plots of intermediate (CD14⁺CD16⁺) and non-classical monocytes (CD14^–CD16⁺). Frequency of both cell types was higher in periodontitis-affected tissue compared to matched controls (n = 21; p < 0.05). (C,D) Representative counter plots and frequency of HLA-DR cells which are gated from live, CD45⁺Lin^–CD16⁺ cells in periodontitis-affected gingival tissue and healthy gingival tissue (n = 21). Frequency of CD45⁺Lin^–CD16 + HLA-DR⁺ cells was significantly higher in periodontitis-affected tissue compared to health (p < 0.05). (E,F) Representative counter plots (values indicate percentage of parent population) and frequency of HLA-DR cells which are gated from live, CD45⁺Lin^–CD14⁺ cells in the periodontitis-affected gingival tissue and healthy gingival tissue (n = 21). Frequency of CD45⁺Lin^–CD14⁺HLA-DR⁺ cells was significantly higher in periodontitis-affected tissue compared to health (p < 0.05). Data presented as mean ± SEM and p less than 0.05 was defined as significant (the symbol “*” indicates p < 0.05 compared to the healthy control).