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. 2020 Feb 26;11:330. doi: 10.3389/fimmu.2020.00330

FIGURE 2.

FIGURE 2

Single cell suspensions from matched periodontitis-affected and healthy gingival tissues were prepared and stained for UV Zombie, CD45, Lin, HLA-DR, CD16, CD14, and PDL1. (A–C): Representative counter plots of CD14+PDL1+ and CD16+PDL1+ cells. Frequency of both cell types was higher in periodontitis-affected tissue compared to matched controls (n = 14; p < 0.05). (D,E): Representative counter plots of intermediate and non-classical monocytes based on HLA-DR and PDL1 expression. HLA-DR+ PDL1+ population was only detectable in intermediate and not in non-classical monocytes. (F) Flow cytometry histogram of FITC-labeled CD47 expression in monocytes in periodontitis-affected tissue (black line) compared to healthy tissue (red line). The histogram was normalized to the number of events and depicts increase in CD47 in periodontitis-affected sites. Data presented as mean ± SEM and p less than 0.05 was defined as significant (the symbol “*” indicates p < 0.05).