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. 2020 Feb 26;11:330. doi: 10.3389/fimmu.2020.00330

FIGURE 3.

FIGURE 3

Single cell suspensions from matched periodontitis-affected and healthy gingival tissues were prepared and stained for UV Zombie, CD45, Lin, CD64, MHC II, CD80, CD163, CD206, PDL1, CD47. (A,B) Representative counter plots of M1 macrophages (CD80+MHC II+). Frequency of M1 macrophages was higher in periodontitis-affected tissue compared to controls (n = 21 pairs; p < 0.05). (C,D) Representative counter plots of M2 macrophages (CD206+CD163+). Frequency of M2 macrophages was lower in periodontitis-affected tissue compared to controls (n = 6 pairs; p < 0.05). (E,F) Flow cytometry histogram of CD47 (FITC) and PDL1 (Qdot 655-A) expression in M1 macrophages in periodontitis-affected tissue (red line) compared to the healthy tissue (black line) shows higher expression of both markers in periodontitis-affected sites. The histogram was normalized to the number of events. (G,H) Flow cytometry histogram of CD47 (FITC) and PDL1 (Qdot655-A) expression in M2 macrophages in periodontitis-affected tissue (black line) compared to the healthy tissue (red line) shows change in both markers in periodontitis affected sites in T2DM (the symbol “*” indicates p < 0.05 compared to the healthy control).

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