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. 2020 Feb 26;11:330. doi: 10.3389/fimmu.2020.00330

FIGURE 4.

FIGURE 4

Single cell suspensions from matched periodontitis-affected and healthy gingival tissues harvested from patients with T2DM were prepared and stained for UV Zombie, CD45, Lin (B220, CD19, Gr1 and Ter119), HLA-DR, CD16, CD14. (A–C) Representative counter plots of intermediate (CD14+CD16+) and non-classical monocytes (CD14–CD16+). Frequency of intermediate monocytes was significantly higher in periodontitis-affected tissue compared to matched controls (n = 6; p < 0.05). (D) Flow cytometry histogram of CD47 (FITC) and PDL1 (Qdot655-A) expression in M2 macrophages in periodontitis-affected tissue (red line) compared to the healthy tissue (black line) shows higher PDL1 expression in periodontitis affected sites in T2DM (the symbol “*” indicates p < 0.05 compared to the healthy control).