FIGURE 5.

Single cell suspensions from matched periodontitis-affected and healthy gingival tissues from T2DM patients were prepared and stained for UV Zombie, CD45, Lin, CD64, MHC II, CD80, CD163, CD206, PDL1, CD47. (A,B) Representative counter plots of M1 macrophages (CD80⁺MHC II⁺). Frequency of M1 macrophages was higher in periodontitis-affected tissue compared to control tissue in T2DM (n = 6 pairs; p < 0.05). (C,D) Representative counter plots of M2 macrophages (CD206⁺CD163⁺). Frequency of M2 macrophages was lower in periodontitis-affected tissue compared to control tissue in T2DM (n = 6 pairs; p < 0.05). (E,F) Flow cytometry histogram of CD47 (FITC) and PDL1 (Qdot 655-A) expression in M1 macrophages in periodontitis-affected tissue (red line) compared to the healthy tissue (black line) shows higher expression of both markers in periodontitis-affected sites in T2DM. The histogram was normalized to the number of events. (G,H) Flow cytometry histogram of CD47 (FITC) and PDL1 (Qdot655-A) expression in M2 macrophages in periodontitis-affected tissue (red line) compared to the healthy tissue (black line) shows change in both markers in periodontitis affected sites in T2DM (the symbol “*” indicates p < 0.05 compared to the healthy control).