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. Author manuscript; available in PMC: 2020 Mar 12.
Published in final edited form as: Cell Rep. 2020 Jan 14;30(2):541–554.e5. doi: 10.1016/j.celrep.2019.12.011

Figure 7. Sterically Blocking the AR-SLNCR1 Interaction Prevents SLNCRI-Mediated Melanoma Invasion.

Figure 7.

(A) Sequences of FANA-modified steric-blocking oligonucleotides used in these assays.

(B) REMSA, as in Figure 1B, of the indicated probes incubated with and without 600 nM of recombinant AR NTD. Steric-blocking competitor oligonucleotides were added to a final concentration of 10 μM.

(C) Matrigel invasion assays of A375 cells transfected with either an empty vector or a vector expression SLNCR1. The indicated FANA-modified oligonucleotides were added 24 h post-transfection, at a final concentration of 500 nM. Invasion is calculated as the percent of invading cells compared to mobile cells as counted in eight fields of view. Top panels show representative images of the indicated invading and mobile cells. Quantification from three independent replicates, represented as mean ± SD, is shown at the bottom. Significance compared to the vector and scramble only control was calculated using the Student’s t test: p < 0.05; **p < 0.005; ***p < 0.0005; ns, not significant.

See also Figure S7.