KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Monoclonal ANTI-FLAG® M2 antibody | Sigma-Aldrich | Cat# F3165, RRID:AB_259529 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| recombinant DDDK-AR protein | abcam | Cat#ab82609 |
| recombinant N-terminal AR-6xHIS protein | RayBiotech | Cat#RB-14-0003P |
| 5-nitroisatoic anhydride | Millipore Sigma | Cat#S428396 CAS#4693-02-1 |
| Dimethyl Sulfate | Millipore Sigma | Cat#D186309 CAS#77-78-1 |
| TRIzol Reagent | Thermo Fisher Scientific | Cat#15596026 |
| Recombinant RNasin Ribonuclease Inhibitor | Promega | Cat#N2511 |
| Recombinant DNase I, RNase-free | Roche | Cat#04716728001 |
| TURBO DNase | Thermo Fisher Scientific | Cat#AM2238 |
| SuperScript II Reverse Transcriptase | Thermo Fisher Scientific | Cat#18064014 |
| Q5 Hot-start high Fidelty DNA Polymerase | New England Biolabs | Cat#M0493 |
| Proteinase K, Lyophilized | Millipore Sigma | Cat#70663 |
| Lipofectamine 2000 Transfection Reagent | Thermo Fisher Scientific | Cat#11668019 |
| DMEM, high glucose, no glutamine | Invitrogen | cat# 11960044 |
| GIBCO FBS | Thermo Fisher Scientific | cat# 26140 |
| Critical Commercial Assays | ||
| PD-10 Desalting Columns | GE Healthcare Life Sciences | Cat#17-0851-01 |
| Agencourt RNA Clean XP kit | Beckman Coulter | Cat#A63987 |
| Agencourt AMPure XP kit | Beckman Coulter | Cat#A63881 |
| Illustra Micro-Spin G50 Columns | GE Healthcare Life Sciences | Cat#27533001 |
| LightShift Chemiluminescent RNA EMSA (REMSA) Kit | Thermo Fisher Scientific | Cat#20158 |
| iScript™ cDNA synthesis kit | BioRad | Cat#1708890 |
| RNeasy Mini Kit | QIAGEN | Cat#74104 |
| Human Total RNA Master Panel II | Clontech | Cat#636643 |
| Fisher HealthCare PROTOCOL Hema 3 Fixative and Solutions | Fisher Scientific | Cat#23-123869 |
| Deposited Data | ||
| The Human Protein Atlas | Uhlén etal., 2015 | www.proteinatlas.org |
| Sequencing Reads and MaP Profiles | Generated in this study | GEO Accession#GSE140048 |
| Experimental Models: Cell Lines | ||
| A375 | American Type Culture Collection | cat# ATCC CRL-1619; RRID:CVCL_0132 |
| WM1976 human melanoma cell line | Wistar Institute | WM1976 |
| HEK293T/17 | American Type Culture Collection | ATCC Cat# CRL-11268, RRID:CVCL_1926 |
| Oligonucleotides | ||
| All Oligonucleotides | See Table S1, this study | N/A |
| Recombinant DNA | ||
| pEGFP-C1-AR | Stenoien et al., 1999; Addgene | Cat#28235 |
| pCDNA3.1(−)+SLNCR1 | Schmidt et al., 2016; Addgene | Cat#86816 |
| Software and Algorithms | ||
| ShapeMapper2 | RNA. 2018 Feb;24(2):143–148. https://doi.org/10.1261/rna.061945.117 | https://github.com/Weeks-UNC/shapemapper2 |
| ΔSHAPE | Biochemistry. 2015 Nov 24;54(46):6867 75. https://doi.org/10.1021/acs.biochem.5b00977 | http://weeks.chem.unc.edu/software.html |
| SuperFold | Nat Protoc. 2015 Nov;10(11):1643–69. https://doi.org/10.1038/nprot.2015.103 | http://weeks.chem.unc.edu/software.html |
| MEME Suite | Bailey et al., 2009 | http://meme-suite.org |
| GraphPad Prism version 7.01 for Windows | GraphPad Software | https://www.graphpad.com/ |
| Other | ||
| 5% Mini-PROTEAN® TBE Gel, 10 well, 30 μl | BioRad | Cat#456501S |
| Series S Sensor Chip CM5, pack of 1 | GE Healthcare | Cat#29104988 |
| Corning BioCoat Matrigel Invasion Chamber with Corning Matrigel Matrix | Fisher Scientific | Cat#S54480 |