Fig 3. Ror is required for normal dendrite regeneration in ddaC and ddaE neurons.

(A) Diagram of the Ror gene and protein with 26-bp deletion resulting in null Ror^Δ26 mutant. The target regions of RNAi hairpins used in this work are mapped onto the gene. Domains in the protein include fz-like CRD, KD, TM, and TK. (B) Representative ddaC dendrite regeneration over the course of 72 HPD. The mutant line was Ror^Δ26/^Δ26;ppk-Gal4, UAS-EB1-TagRFP-T crossed to Ror^Δ26/^Δ26. Control flies used were yw;ppk-Gal4, UAS-EB1-TagRFP-T crossed to yw. Each neuron was imaged every 24 h to quantify regeneration over time in yw (control) or Ror^Δ26 null mutants. Quantitation is shown in (C). (D) Representative dendrite regeneration over time in ddaE neurons. Dorsal comb dendrites were severed (red arrowhead), and new branchpoints (green arrowheads) were counted at 24 h intervals. Mutant phenotype: Ror^Δ26/^Δ26;221-Gal4, UAS-mCD8-GFP crossed to Ror^Δ26/^Δ26. Control flies used were yw;221-Gal4, UAS-mCD8-GFP crossed to yw, and quantitation is shown in (E). Graphs show mean regeneration diameter, with error bars showing standard deviation (C) or added branch points (E). Sample size (within bar, bottom) represents individual neurons from each animal. *P < 0.05, **P < 0.01, ***P < 0.001 with Mann–Whitney U test to compare mean rank. Quantitation is contained in S1 Data. CRD, cysteine-rich domain; da, dendritic arborization; ddaC/E, dorsal da C/E; EB1, end binding protein 1; fz, frizzled; GFP, green fluorescent protein; HPD, hours postdendrotomy; KD, kringle domain; ppk, pickpocket; RFP, red fluorescent protein; RNAi, RNA interference; Ror, RTK-like orphan receptor; RTK, receptor tyrosine kinase; TK, tyrosine kinase domain; TM, transmembrane domain; yw, yellow, white.