Fig 8. Prediction of experimentally measured epistasis by DCA from [12].

A: Scatter plot of average epistasis magnitude $|\Delta \Delta \mathcal{G}|$ vs DCA-inferred energetic couplings $|\Delta \Delta \mathcal{E}|$, where the color code distinguishes short and long distance pairs of residues on the PDZ α2-helix three-dimensional structure. ρ, the Pearson correlation coefficient, indicates a better performance at short range. As a comparison, in B we show the scatter plot of average epistasis magnitude $|\Delta \Delta \mathcal{F}|$ vs DCA-inferred energetic couplings $|\Delta \Delta \mathcal{E}|$ in our in silico evolved networks: similarly to A, the prediction at long distance is poorer than at short distance.