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ZupT is required for growth during metal limitation. Growth curves of WT C. difficile, mutant zupT::CT, and complemented zupT::CT pJS116_zupT grown in the presence of 0.35 mg/ml calprotectin (A) or 50 μM TPEN (C). Percent growth based off maximum OD at 6 h is shown for calprotectin (B) and TPEN (D). Statistical significance was determined by t test (*, P < 0.05).
