TABLE 1.
Bacterial strains and plasmids used in this study.
| Bacterial strain or plasmid | Relevant feature or genotype | Reference |
|---|---|---|
| Clostridioides difficile R20291 | (29) | |
| Clostridioides difficile zupT::CT | Intron inserted into zupT | This study |
| Bacillus subtilis JH BS2 | Carries Tn196 | (28) |
| Escherichia coli DH5α | (30) | |
| Escherichia coli MG1655 | RecA+ | (31) |
| pJS107 | ClosTron plasmid | (28) |
| pJS107_zupT | ClosTron plasmid with intron targeted to zupT | This study |
| pJS116 | Stable C. difficile plasmid | (28) |
| pJS116_pzupT-zupT | zupT::CT complementation plasmid | This study |