TABLE 2.
Colistin MICs of strains generated in this studya
| Strain | Colistin MIC (μg/ml)b |
|---|---|
| BW25113 | 0.25 |
| BW27848 | 0.125 |
| BW25113::Tn7 empty | 0.25 |
| BW27848::Tn7 empty | 0.125 |
| BW27848::Tn7 BW25113 | 0.125 |
| BW27848::Tn7 I1121 | 2 |
| BW27848::Tn7 I1121m | 0.25 |
| BW27848::Tn7 H2129 | 4 |
| BW27848::Tn7 H2129m | 0.25 |
| BW27848::Tn7 G821 | 4 |
| BW27848::Tn7 G821m | 0.25 |
| BW27848::Tn7 F2745 | 0.25 |
| BW27848::Tn7 E3090 | 0.125 |
| BW27848::Tn7 E2372 | 0.25 |
| BW27848::Tn7 D2373 | 0.5 |
| BW27848::Tn7 A2361 | 0.125 |
| BW27848::Tn7 Z821 | 2 |
| BW27848::Tn7 Z821m | 0.125 |
a
E. coli strain BW27848 is the ΔbasRS mutant of BW25113 (49). The basRS alleles of colistin-resistant strains from this study were inserted into the attTn7 site of BW27848. The addition of “m” to a strain name indicates that the construct has been modified through inverse PCR site-directed mutagenesis to reverse the mutation associated with colistin resistance.
b
The values represent the medians from three independent replicate experiments performed in triplicates.