FIG 5.

Microscopic analysis of filament formation in Candida auris rad9, mrc1, and rad51 mutants (UACa11 background). (A) Representative microscopy images of C. auris filaments after growth of wild-type (UACa11), rad9Δ, mrc1Δ, and rad51Δ cells in YPD broth with or without the addition of the indicated drug for 18 to 20 h at 30°C. (B) Representative microscopic images of C. auris filaments after growth of wild-type (UACa11), rad9Δ, mrc1Δ, and rad51Δ cells on YPD plates containing 100 mM HU after 3 days at 30°C. In panels A and B, bright-field images are shown in the left columns, and merged fluorescent images (chitin stained by calcofluor white [CFW] and DNA stained by SYBR green I) are shown in the right columns. Scale bar, 10 μm. (C) Length of filaments observed after growth of wild-type (UACa11) (n = 150), rad51Δ (n = 50), rad9Δ (n = 50), and mrc1Δ (n = 50) cells in YPD broth containing 100 mM HU for 18 to 20 h at 30°C. Only cells longer than 6 μm were considered filaments. The dotted line indicates average length of 300 yeast cells (wild-type UACa11 grown in YPD for 18 to 20 h at 30°C). *, P < 0.05, for results relative to those of the wild type (UACa11) (Wilcoxon rank sum test); ns, not significant. (D) Length of filaments observed after growth of rad51Δ (n = 50) and mrc1Δ (n = 50) cells in YPD medium for 18 to 20 h at 30°C without genotoxic stress. Only cells longer than 6 μm were considered filaments. The dotted line indicates average length of 300 yeast cells (wild-type UACa11 grown in YPD for 18 to 20 h at 30°C).