KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Monoclonal mouse anti-ELACI | Santa Cruz | Cat#sc-390029 |
| Polyclonal rabbit anti-TRNT1 | Novus Biologicals | Cat#NBP1–86589; RRID: AB_11016077 |
| Polyclonal rabbit anti-ELAC2 | Bethyl Laboratories | Cat#A304–775A; RRID: AB_2620970 |
| Monoclonal mouse anti-ANKZF1 | Santa Cruz | Cat#sc-398713 |
| Monoclonal mouse anti-Flag M2 | Sigma | Cat#3165; RRID: AB_259529 |
| Polyclonal rabbit anti-GFP | homemade | N/A |
| Polyclonal rabbit anti-RFP | homemade | N/A |
| Monoclonal rabbit anti-uL2 | Abcam | Cat#ab169538; RRID: AB_2714187 |
| HRP-conjugated goat anti-rabbit | Jackson ImmunoResearch | Cat#111-035-003; RRID: AB_2313567 |
| HRP conjugated goat anti-mouse | Jackson ImmunoResearch | Cat#115-035-003; RRID:AB_10015289 |
| Bacterial and Virus Strains | ||
| BL21 (DE3) competent cells | Invitrogen | Cat#C600003 |
| DH5α competent cells | Invitrogen | Cat#18265017 |
| Rosetta2 (DE3) competent cells | Millipore | Cat#71400 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| TRNT1 | Yip et al., 2019 | N/A |
| ELAC1 | This study | N/A |
| ELAC1 (H64A) | This study | N/A |
| ELAC2 (31–826) | This study | N/A |
| ELAC2 (31–826, H548A) | This study | N/A |
| Trz1p | This study | N/A |
| Vms1p | Yip et al., 2019 | N/A |
| DN-Hbs1L | Shao et al., 2013 | N/A |
| Hbs1L | Shao and Hegde, 2014 | N/A |
| Pelota | Shao and Hegde, 2014 | N/A |
| ABCE1 | Shao and Hegde, 2014 | N/A |
| NEMF | Shao et al., 2015 | N/A |
| RtcB | New England Biolabs | Cat#M0458 |
| CNP | This study | N/A |
| T4 PNK | New England Biolabs | Cat#M0201 |
| T7 RNA polymerase | homemade | N/A |
| Creatine phosphate | Roche | Cat#621714 |
| Creatine kinase | Roche | Cat#127566 |
| SuperTEV | homemade | N/A |
| Ulp1 SUMO protease | homemade | N/A |
| 3C protease | homemade | N/A |
| Ni-NTA agarose | QIAGEN | Cat#30250 |
| Glutathione Sepharose 4B | GE Healthcare | Cat#17–0756 |
| DEAE Sepharose Fast Flow | GE Healthcare | Cat#17–0709 |
| Phenyl Sepharose 6 Fast Flow | GE Healthcare | Cat#17–0973 |
| HiTrap Heparin HP affinity column | GE Healthcare | Cat#17040601 |
| L-Glutathione reduced | Sigma | Cat#G4251 |
| Cycloheximide | CalBiochem | Cat#239763 |
| Puromycin dihydrochloride | GIBCO | Cat#A1113803 |
| [α-33P]-CTP | American Radiolabeled Chemicals | Cat#ARP0154 |
| TRI Reagent | Zymo Research | Cat#R2050–1 |
| rRnasin | Promega | Cat#N2511 |
| Complete EDTA-free protease inhibitor cocktail | Roche | Cat#11873580001 |
| TransIT 293 | Mirus | Cat#MIR2706 |
| SYBR Gold | Invitrogen | Cat#S11494 |
| Hydrogen peroxide | Sigma | Cat#216763 |
| S. cerevisiae genomic DNA | Novagen | Cat#69240–3 |
| M2 Flag affinity resin | Sigma | Cat#A2220 |
| 3× Flag peptide | Sigma | Cat#F4799 |
| Rabbit reticulocyte translation system | Feng and Shao, 2018 | N/A |
| Experimental Models: Cell Lines | ||
| HEK293T | ATCC | Cat#CRL-11268 |
| ELAC1 KO sg1–2 293T | This study | N/A |
| ELAC1 KO sg1–5 293T | This study | N/A |
| ELAC1 KO sg2–3 293T | This study | N/A |
| ELAC1 KO sg2–9 293T | This study | N/A |
| ANKZF1 KO sg1–3 293T | This study | N/A |
| ELAC1/ANKZF1 DKOsg1–1 293T | This study | N/A |
| ELAC1/ANKZF1 DKO sg1–2 293T | This study | N/A |
| Oligonucleotides | ||
| ELAC1 sg2 TAAAGTCCCGAAGCCCTACA | This study | N/A |
| ELAC1sg1 AGCACTCGCCTTCACACCGA | This study | N/A |
| ANKZF1 sg1 GCTTGGCCCGAACCGTATAG | This study | N/A |
| Recombinant DNA | ||
| pRSETA-TRNT1 | Yip et al., 2019 | N/A |
| pGEX-ELAC1 | This study | N/A |
| pGEX-ELAC1 (H64A) | This study | N/A |
| pK27SUMO-ELAC2 (31–826) | This study | N/A |
| pK27SUMO-ELAC2 (31–826, H548A) | This study | N/A |
| pK27SUMO-Trz1 | This study | N/A |
| pLeuUUA-HDV-ΔCCA | Yip et al., 2019 | N/A |
| pX459-sgELAC1–1 | This study | N/A |
| pX459-sgELAC1–2 | This study | N/A |
| pRSETA-CNP | This study | N/A |
| pSP64–3XFlag-VHP68 | Shao et al., 2013 | N/A |
| pX459-sgANKZF1–1 | This study | N/A |
| pcDNA3.1–3XFlag-ELAC1 | This study | N/A |
| pcDNA3.1–3XFlag-ELAC1 (H64A) | This study | N/A |
| pcDNA3.1–3XFlag-ANKZF1 | Yip et al., 2019 | N/A |
| pcDNA5-FRT/TO-GFP-P2A-repA0-P2A-RFP | Juszkiewicz and Hegde, 2017 | N/A |
| pcDNA5-FRT/TO-GFP-P2A-repA63-P2A-RFP | Juszkiewicz and Hegde, 2017 | N/A |