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. Author manuscript; available in PMC: 2020 Aug 17.
Published in final edited form as: Nat Struct Mol Biol. 2020 Feb 17;27(3):240–248. doi: 10.1038/s41594-020-0380-1

Extended Data Fig. 5. FANCD2 dimers cannot be ubiquitinated and exchange with FANCI to form a D2–I heterodimer.

Extended Data Fig. 5

a Monoubiquitination assays of D2–I and FANCD2 homodimer. The FANCD2 homodimer had a StrepII-tag and was therefore larger than D2 in the D2–I complex. Monoubiquitination efficiency was analyzed by Coomassie blue SDS-PAGE (top) and Western blotting the His-tagged ubiquitin (bottom). b Exchange assay. The FANCD2 homodimer was immobilized on Streptactin resin and incubated with free FANCI. The resin was washed, then bound and unbound fractions were analyzed by SDS-PAGE. These data are representative of experiments performed twice. Uncropped images are available in Supplementary Fig. 1.