Effect of NL-1 and NL-1 nanoparticles on apoptosis in bEnd.3 cells following ischemia and reperfusion. Figures A-D are
representative flow cytometry data for cell blank (A), 3 hour OGD (B), and 3 hour OGD with NL-1 (C) and 3 hour OGD with NL-1
nanoparticles (D). Each figure shows four quadrants: live (bottom left), early apoptotic phase (bottom right), late apoptotic
phase (top right), and necrotic (top left) cell populations. Results are indicated in terms of improvement in cell survival at 3
hours (E) and 6 hours (F) ischemic period, as well as the reduction in late stage apoptosis after 3 hours (G) and 6 hours (H) of
ischemia (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001).