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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: J Struct Biol. 2020 Feb 4;210(1):107474. doi: 10.1016/j.jsb.2020.107474

Fig. 1. On-grid cell culture set up and confocal imaging for on-grid validation.

Fig. 1.

(A) Bright-field image of MDA-MB231 cells seeded on a fibronectin-functionalized carbon-coated gold TEM grid in a cell culture dish (arrow represents location of cells on the suspended fibronectin-coated carbon support film). (B) Bright-field image and (C) fluorescence confocal image of MDA-MB231 cells grown uniformly on the grid, including in the electron-transparent grid squares. Blue – DAPI (nuclei), green-Phalloidin Alexa Fluor 488 (F-actin).