Table 2.
Effect of PACAP38 and GnRH on the gonadotropic hormone level and gene expression in cell cultures.
| Type of Cell Cultures | Examined Parameters | ||||
|---|---|---|---|---|---|
| Perifused cell culture | α-subunit | LH | FSH | ||
| cPACAP | ↑ | – | ↑ | (126, 127) | |
| pPACAP | ↑ | ↑ | – | (63, 129) | |
| Primary cell culture | α-subunit | α-subunit | (128) | ||
| mRNA | protein | ||||
| PACAP | ↑ | ||||
| GnRH | ↑↑ | ↑↑ | |||
| αT3-1 cell line | (128) | ||||
| PACAP | ↑ | ↑ | |||
| GnRH | ↑ | ↑ | |||
| Primary cell culture | α-subunit | LHβ | FSHβ | PAC1 | (122) |
| promoter activity | |||||
| cPACAP | ↑ | ||||
| cGnRH | ↑ | ↑ | ↑ | ||
| mRNA | |||||
| cPACAP | ↑ | ↑ | ↑ | ||
| CGnRH + PACAP | ↑ | ↑ | ↑ | ||
| perifused Lβ-T2 | |||||
| pPACAP high f | ↑↑ | ↑ | ↑ | ||
| pPACAP low f | ↑ | ↑↑ | ↑↑ | ||
| GnRH | ↑ | ||||
P, pulsatile; c, continuous; f, frequency.
↑ moderate increase, ↑↑ high increase.