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. 2020 Mar 12;11:1326. doi: 10.1038/s41467-020-15125-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Western blot analysis probed with FHR antiserum (αFHR) of whole-cell lysates from FHR^+/+ and FHR^−/− slender BSFs, induced stumpy BSFs, and PCFs in culture and from FHR^+/+ stumpy BSFs from a mouse infection. Anti-PFR (αPFR) was used as a loading control. b, c Live-cell-binding assay of 100 nM fluorescent b bovine FH or c FHR-moAb to PCFs in culture. After 2 h, cells were fixed in culture and visualized. Scale bar, 10 μm. d Immunofluorescence analysis of localization of FHR during the majority of the T. brucei life cycle. Cells were collected, fixed and then probed with FHR antiserum. Scale bar, 10 μm. DNA, Hoechst staining of the nuclear and kinetoplastid DNA. Cells positioned with the posterior towards the top right corner. Bars beneath the microscope images represent where complement is present during the life cycle (yellow gradient) and FHR expression (blue gradient). Source data are provided as a Source Data file.