Fig. 6. The transmission of T. brucei FHR^−/− cell lines to tsetse flies from mice was significantly reduced compared with FHR^+/+.

a, b Mean mouse blood parasitaemia of parental FHR^+/+ and two independent FHR^−/− clones, with error bars representing the SEM. a Five immunosuppressed BALB/c mice were infected with each cell line and monitored from days 3–14 post infection. b Five immunocompetent BALB/c mice were infected with each cell line and monitored from days 2–24 post infection. c Tsetse flies were fed on infected mice and transmission of the infection was evaluated by the dissection of >100 midguts per cell line on days 6–16 post infection. The total number is derived from seven replicates per cell line. The number of infected midguts over the total dissected midguts is plotted, whereby 31/114 were infected for FHR^+/+, 12/119 for FHR^−/− clone 1, and 11/106 for FHR^−/− clone 2. The observed difference between the FHR^+/+ and each of the FHR^−/− clones was significant (P < 0.002 for each cell line by χ²-test). Source data are provided as a Source Data file.