Table 4.
Effect of DCR subculture medium on TDZ-induced adventitious buds/shoot.
| PGRs(μM) | Buds induction(%) | Available shoots/ explant | Explants status | ||
|---|---|---|---|---|---|
| TDZ | BA | NAA | |||
| 9.08 | 0.00 | 0.27 | 100.0 ± 0.0A | 8.9 ± 0.7B | Callus loosen, granular and adventitious bud points on surface; bud, leaf deformity. |
| 0 0.00 | 0.00 | 0.00 | 100.0 ± 0.0A | 7.6 ± 0.5C | Shoot, leaf grow normal, bud quality generally. |
| 0.00 | 22.20 | 0.27 | 100.0 ± 0.0A | 35.2 ± 0.6A | Callus densification, differentiate into tufted, robust bud; bud, leaf grow normal. |
Note: Value represents the mean ± SE of 3 independent experiments with 40 explants per treatment. Data in the same column followed by different letters are significantly different (Duncan’s test at P < 1% level).