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. 2020 Mar 12;10:4572. doi: 10.1038/s41598-020-61663-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Separation of fibrils by ThT fluorescence intensity. (A) ThT fluorescence emission intensities of twenty MoPrP fibril samples prepared under identical conditions. The samples are grouped into three intensity regions, with some samples having low (grey), medium (blue) and high (red) emission intensities. (B) Seeded aggregation kinetics of the three intensity region fibrils using 10% of low, medium and high intensity fibrils. (C) Fluorescence intensity “drops” at the early stages of the aggregation process.