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. 2020 Mar 12;10:4621. doi: 10.1038/s41598-020-61309-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transmission electron microscopy and immuno-gold labeling of platelet histones. Cells were immuno-gold labeled with anti-histone 4 antibodies and then imaged with transmission electron microscopy. (A,B) Platelets are shown to label positive for histone 4 in their cytoplasm as well as on their cell membrane. Magnified images are shown in the bottom panels (Aii and Bii). (C) HT-29 cells were also immuno-labeled and imaged as a positive control and showed nuclear labeling of histone 4. Magnification of the nuclear envelope with labeling is shown on the bottom panel (Cii). Red arrows point towards immuno-gold particles (6 nm).