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. 2020 Mar 12;10:4593. doi: 10.1038/s41598-020-58126-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Direct effect of mesenchymal stem cells (MSCs) on lymphocytes. (a) Representative mixed lymphocyte reaction data for lymphocyte (lymp), lymphocyte + soluble factors from MSCs (lymp + SF), and lymphocyte + MSC (lymp + MSC) groups. The graph shows the mitotic index in each group (n = 3, each). (b) Representative flow cytometry data for lymp, lymp + SF, and lymp + MSC groups stained with anti-CD4 and anti-CD8 antibodies. The graph shows the ratio of CD8 + cells to CD4 + cells in each group (n = 3, each); ***P < 0.001. (c) Representative flow cytometry data for lymp, lymp + SF, and lymp + MSC groups stained with anti-CD4, anti-IL-4, and anti-IFN-g antibodies. The graph shows the percentage of IL4-IFG-g + cells among CD4 + cells in each group (n = 3, each); ***P < 0.001. (d) The concentration of cytokines in lymp, lymp + SF, and lymp + MSC groups (n = 3, each); *P < 0.05, **P < 0.01, ***P < 0.001.