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. 2020 Mar 12;10:4556. doi: 10.1038/s41598-020-61279-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Histological structure of cecal mucosa (A) and PPs in midpiece colon (B) in 42-day old piglets (stained with H&E). In all panels, the structures are indicated by LP, Lamina propria; MM, muscularis mucosae; MW, muscular wall; PPs, Peyer’s Patches; and S, Submucosa. (A1–A3) Abundant lymphocytes were visible in the lamina propria of ceca from a Duan-Nai-An-treated piglet (A1), a yeast-treated piglet (A2), and a control piglet (A3). (B1) In the Duan-Nai-An-treated piglet, PPs were well-developed in the submucosa of midpiece colon and there were abundant lymphocytes in the lamina propria. (B2) In the yeast-treated piglet, PPs were well-developed in submucosa of midpiece colon. (B3) In the control piglet, PPs were atrophied in the submucosa of midpiece colon and there were congestion in lamina propria.