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. 2020 Mar 12;10:4556. doi: 10.1038/s41598-020-61279-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Histological structure of colonic mesenteric lymph nodes in 42-day old piglets (stained with H&E). In all panels, Co indicates cortex; LF indicates lymphoid follicle; M indicates medulla. (A) In the Duan-Nai-An-treated piglet, the medulla of lymph nodes or PPs were well-developed with some lymphoid follicles proliferated into the cortex, and germinal centers were obvious. (B) In the yeast-treated piglet, the medulla of lymph nodes (or PPs) were well-developed and lymphocytes were dense; (C) In the control piglet, the lymph nodes (or PPs) were atrophied and lymphocytes were sparse.