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. 2020 Mar 12;10:4556. doi: 10.1038/s41598-020-61279-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Electron microscopic micrographs of the duodenum in 42-day old piglets. (A1) SEM of the duodrenal mucosa of a Duan-Nai-An-treated piglet showing strong and thick villi. (A2) SEM of the duodenal mucosa of a yeast-treated piglet showing strong and thick villi and mucus residues. (A3) SEM showing the duodenal mucosa of a control piglet with villi sloughed off. (B1) TEM of the duodenal villi of a Duan-Nai-An-treated piglet. The microvilli were structurally intact and densely oriented (indicated by an arrow). (B2) TEM of the duodenal villi of a yeast-treated piglet. Microvilli were partially fractured (indicated by an arrow). (B3) TEM of the duodenal villi of a control piglet. Microvilli sloughed off (indicated by an arrow). (C1) TEM showing a large number of lymphocytes (indicated by an arrow) in the duodenal lamina propria of a Duan-Nai-An-treated piglet. (C2) TEM showing a large number of lymphocytes (indicated by an arrow) in the duodenal lamina propria of a yeast-treated piglet. (C3) TEM of the duodenal lamina propria of a control piglet showing necrosis, karyopyknosis and chromatin margination of lymphocytes (indicated by △).