Fig. 2. Stat1- and Cdkn2a-dependent induction of the senescence markers pHP1γ, H3K9me3, and of SA-β-gal in transplanted RT2-cancers.
a–d Representative microscopic images of s.c. RT2-cancers from either RT2-, RT2.Stat1−/−-, RT2.Cdkn2a−/−- or from RT2.CRISPR-Cdkn2a-cancer cells. Mice were treated with isotype control mAbs (Ctr) or with immune checkpoint blockade (ICB, anti-PD-L1 and anti-LAG-3). Staining for the senescence marker p21Cip1 (red), the proliferation marker Ki67 (blue) and for nuclei (white) (a), pHP1γ (red), nuclei (white) (b), H3K9me3 (red), nuclei (white) (c). SA-β-gal activity at pH5.5 and percentage of SA-β-gal positive tumour cells in each tumour (d). The colour evaluation and calculation of the SA-β-gal+ cells are described in Supplementary Fig. 2 and Methods (for d). Scale bars 10 µm (a), 2 µm (b, c), 1000 µm (d). Histology was performed in one to three representative tumours from Fig. 1c.