Fig. 6. Stat1-dependent induction of the nuclear senescence markers p21^Cip1, HP1γ, H3K9me3, and of SA-β-gal in RT2-cancers by combined ICB/AT therapy.

Representative fresh frozen cryostat sections of RT2-cancers from either RT2 or RT2.Stat1^−/− mice with established cancers were treated with either isotype control mAbs (Ctr), with adoptive transfer of TAA-specific T_H1 cells (AT), with immune checkpoint inhibitors (ICB; anti-PD-L1 and anti-LAG-3), or with ICB/AT. a p21^Cip1 (red), Ki67 (blue), nuclei (green). b HP1γ (red), nuclei (white). c H3K9me3 (red), nuclei (white). d Representative microscopic images of SA-β-gal activity at pH5.5 and percentage of SA-β-gal positive tumour cells in each tumour. The colour evaluation and calculation of the SA-β-gal⁺ cells are described in Supplementary Fig. 2 and Methods. Scale bars 2 µm (a–c), 1000 µm (d). Histology was performed in one to three representative tumours from Fig. 4e.