Figure 3.

Fluorescence polarization emission spectra of ciprofloxacin-GlnBP-CF647 in the absence and presence of an increasing concentration of anti-ciprofloxacin. Variation of the maximum fluorescence value at 668 nm as a function of the antibody concentration (inset). All the measurements were done in PBS buffer 0.01 M pH 7.4 at 37 °C.