Figure 4.
RvD6s enhance corneal nerve regeneration. (A) Whole-mount images of normal corneal nerves stained with anti-PGP9.5, a pan-marker for total corneal nerves and SP, a major neuropeptide in the mouse cornea. The insets, which are marked by a dashed box in the whole-mount images, show the amplified center area of the cornea with double PGP 9.5 and SP staining, and PGP 9.5 and SP alone. (B,C) Representative wholemount images and calculated nerve density of PGP 9.5 (B) and SP (C) positive axons at 12 days after injury and treatment. Data were normalized to the baseline (uninjured corneas in A). The statistical p-value is derived from one-way ANOVA, followed by Tukey’s honest significant difference (HSD) multiple pairwise comparisons.