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. 2020 Mar 12;11:1345. doi: 10.1038/s41467-020-15180-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Western blot (WB) of the indicated proteins bound to chromatin isolated from SN incubated with mitotic or interphase extracts for the indicated times. b WB of the indicated proteins bound to chromatin isolated from SN pre-incubated with 200 ng/μl recombinant SSRP1 or control buffer and transferred to interphase extracts for the indicated times. SSRP1 input is 10% of the total. c WB of chromatin isolated from interphase egg extracts supplemented with sperm nuclei pre-incubated with buffer (−) or purified histone H1 (+) at a final concentration of 2 μM for 30 min in the presence of 200 ng/μl recombinant SSRP1 or control buffer and probed with the indicated antibodies. d Representative autoradiography of replication time course assay. Sperm nuclei were pre-incubated for 30 min with 200 ng/μl recombinant SSRP1, 2 μM histone H1, or both and then transferred to interphase extracts. Samples were taken at the indicated times after nuclei and α-³²P-dCTP addition to egg extracts. OD for each lane is indicated. Images in all figures show a typical result of experiments repeated at least three times.