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. 2020 Mar 12;10:4618. doi: 10.1038/s41598-020-60751-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Clade date estimation based on maximum likelihood analysis of mtDNA (sequences as in Fig. 2). The dotted lines indicate the dates based on the most probable tree topology. The shaded regions show the effect of taking uncertainty in the tree topology into account by proportionally weighting the dates suggested by the 10 most-probable tree topologies (capturing 93% of probability according to the IQTree bp-RELL method).