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Gene-specific primer and probe sequences used
| Gene | Primera |
|---|---|
| ORF1b | |
| Forward | 5′-TGGGGYTTTACRGGTAACCT-3′ |
| Reverse | 5′-AACRCGCTTAACAAAGCACTC-3′ |
| Probe | 5′-TAGTTGTGATGCWATCATGACTAG-3′b |
| N | |
| Forward | 5′-TAATCAGACAAGGAACTGATTA-3′ |
| Reverse | 5′-CGAAGGTGTGACTTCCATG-3′ |
| Probe | 5′-GCAAATTGTGCAATTTGCGG-3′b |
Y is C or T; R is A or G; W is A or T.
In 5′-6-carboxyfluorescein/ZEN internal quencher/3′-Iowa Black fluorescent quencher format.
