Uterine artery contractility measured by wire myography and the effect of AMPK activation on blunting the contractile response. A) The contractile response to PE was similar in uterine artery from normoxic and hypoxic mice. A769662 blunted the PE response in both B) normoxic and C) hypoxic mice. D) The magnitude of A769662 effects on blunting PE response was similar in both groups. E) Neither the maximum KCl response nor the maximum response to PE was changed by hypoxia or A769662, but sensitivity to PE was reduced by A769662 in vessels from both normoxic and hypoxic groups. The contractile response to PE was normalized to the maximum KCl response in each vessel studied. Data are presented as mean ± SEM and NS = non-significant. Different italicized letters represent significant differences using either the overall result using two-way ANOVA or two-way ANOVA with Sidak multiple comparisons. CON = control untreated. N = Norm CON: 8 mice, 12 vessels; Norm +A769662: 8 mice, 12 vessels; Hx CON: 7 mice, 8 vessels; Hx + A769662: 7 mice, 7 vessels.