Figure 3.

Vasodilatory effect of AMPK activation in preconstricted uterine artery. A) Preconstricted uterine arteries from normoxic and hypoxic mice similarly vasodilated in response to the AMPK activator A769662. B) Incubation with L-NAME reduced the vasodilatory effect of A769662 in uterine arteries from normoxic mice and C) did so to a lesser degree in hypoxic mice. Addition of indomethacin did not further reduce the effect of A769662 in normoxic mice but did so in hypoxic mice. D) Comparison of curves by maximum relaxation, EC₅₀, and AUC. Data are presented as mean ± SEM and normalized to initial PE contraction. Response curves were analyzed by two-way ANOVA. In part D, the overall ANOVAs are shown, and different italicized letters represent results of Sidak multiple comparisons. NS = non-significant. CON = control untreated. N = Norm CON: 7 mice, 9 vessels; Norm +L-NAME: 7 mice, 8 vessels; Norm +L-NAME +INDO: 3 mice, 3 vessels; Hx CON: 7 mice, 8 vessels; Hx + L-NAME: 7 mice, 7 vessels; Hx + L-NAME +INDO: 6 mice, 6 vessels.