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. 2019 Oct 17;102(3):607–619. doi: 10.1093/biolre/ioz202

Figure 3.

Figure 3

Fetal lethality of COXIII-81 chimeras. (A) Heteroplasmy levels (%) and locations of non-synonymous protein-coding mutations in COXIII-81, ND6-100, ND2-50, and ATP8-62 ESC lines. ND indicates that mtDNA mutations were not detected in Control-1 ESC line. (B) Proportion of GFP-positive ESCs in COXIII-81 and chimeric blastocysts (E3.5). ns denotes P ≥ 0.05. (C) Detection of COXIII-81 ESC contribution by GFP in chimeric blastocysts. The upper panel is a bright field image and the lower panel is a fluorescent image. Scale bar = 100 μm. (D) Implantation rate of COXIII-81 and Control-1 chimeric embryos. The implantation rate was calculated as the number of implantation site/number of embryos transferred. ns denotes P ≥ 0.05. (E) Percent of viable E9.5 fetuses in COXIII-81 and Control-1 chimeras. The viable fetus rate was estimated as the number of viable fetuses/number of implantation sites. (F) Morphology of degenerated E9.5 fetus (Fetus #1) from COXIII-81 chimeras. The upper panel shows a bright field image and the lower panel shows a fluorescent image. Scale bar = 1 mm. (G) Heteroplasmy levels of 9138 G > A mutation in mt-CoxIII in degenerated E9.5 fetuses and in live chimeric pups. (H) Heteroplasmy level of 9278 A > T mutation in mt-CoxIII in degenerated E9.5 fetuses and in live chimeric pups.