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Ingredients used in gram/liter in the preparation of M-Staphylococcus broth.
| Ingredients | Gram/liter |
|---|---|
| Casein enzymic hydrolysate | 10.0 |
| Yeast extract | 2.5 |
| Lactose | 2.0 |
| Mannitol | 10.0 |
| Dipotassium hydrogen phosphate | 5.0 |
| Sodium chloride | 75.0 |
| Sodium azide | 0.049 |
| PH (at 25°C) | 7.0 ± 0.2 |
About 104.55 grams of the media was suspended in 1000 ml distilled water. It was mixed thoroughly and heated to boiling for 5 minutes. The media were then autoclaved at 15 lbs at 121°C for 15 minutes.
